By Y. P. S. Bajaj (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
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Extra resources for Plant Protoplasts and Genetic Engineering II
Sample text
Theor Appl Genet 67:499-504 Aviv D, Arzee-Gonen P, Bleichman S, Galun E (1984b) Novel alloplasmic Nicotiana plants by "donor-recipient" protoplast fusion: cybrids having N tabacum or N sylvestris nuclear genomes and either or both plastomes and chondriomes from alien species. Mol Gen Genet 196:244-253 Ayers NM (1987) Inheritance of organelles in somatic cell hybrids and cybrids of Nicotiana. Ph D Thesis, Univ Nebraska, Lincoln 18 y. P. S. Bajaj Bajaj YPS (1974) Isolation and culture studies on pollen tetrad and pollen mother cell protoplasts.
Nature (London) 310:511-514 Brown S (1984) Analysis and sorting of plant material by flow cytometry. Physiol Veg 22:341-349 Brown S, Renaudin JP, Prevot C, Guem J (1984) Flow cytometry and sorting of plant protoplasts: technical problems and physiological results from a study of pH and alkaloids in Catharanthus roseus. Physiol Veg 22:541- 554 Brown S, Jullien M, Coutos-Thevenat P, Muller Ph, Renaudin JP (1986) Present developments of flow cytometry in plant biology. Bioi Cell 58: 173 -178 Burgess J (1984) The revolution that failed.
Further, the genes which enabled the recombination may not be desired in the final population, and may have to be removed again. More promising but even less explored are external agents disturbing normal restriction patterns. Temperature extremes may be a candidate, but these usually merely reduce recombination. In special cases it may be possible to break linkages by splitting a chromosome into two or by the induction of translocations, by irradiation or by inserting an in-vitro callus phase.