By Q.-G. Cai, C.-K. Tsai, Y.-Q. Qian, Y. C. Chien, S.-Q. Ke, Z.-C. He (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
In continuation of Volumes eight, nine, and 22 on in vitro manipulation of plant protplasts, this new quantity offers with the regeneration of vegetation from protoplasts and genetic transformation in a number of species of Actinidia, Amoracia, Beta, Brassica, Cicer, Citrus, Cucumis, Duboisia, Fragaria, Glycine, Ipomoea, Lactuca, Lotus, Lycopersicon, Manihot, Medicago, Nicotiana, Petunia, Phaseolus, Pisum, Prunus, Psophocarpus, Saccharum, Solanum, Sorghum, Stylosanthes, and Vitis. those stories replicate the far-reaching implications of protoplast know-how in genetic engineering of vegetation. they're of specified curiosity to researchers within the box of plant tissue tradition, molecular biology, genetic engineering, and plant breeding.
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Additional info for Plant Protoplasts and Genetic Engineering IV
Example text
Myoporoides (Dm), D. leichhardtii (Dl) and D. hopwoodii (Dh) 7 Conclusion and Prospects Duboisia protoplasts were isolated from suspension cells, regenerated shoots, and cultured roots. Protoplasts isolated from suspension cells of Dm, Dl, and Dh divided and formed colonies in modified B5 medium, although there were differences among species with respect to culture conditions required for colony formation. Colonies developed into calli when two-step culture methods were employed. The calli of Dm and Dl, but not Dh, regenerated shoots which gave rise to plants.
Physiol Plant 21: 930-940 Constabel F (1982) Isolation and culture of plant protoplasts. In: Wetter LR, Constabel F (eds) Plant tissue culture methods. National Research Council of Canada, Saskatoon, pp 38-48 Cosson L, Vaillant JC, Dequeant E (1976) The tropane alkaloids from the leaves of Duboisia myoporoides from New Caledonia. Phytochemistry 15: 818-820 30 Y. Kitamura Coulson JF, Griffin WJ (1967) The alkaloids of Duboisia myoporoides. I. Aerial parts. Planta Med 15:459-466 Coulson JF, Griffin WJ (1968) The alkaloids of Duboisia myoporoides.
Although Agrobacterium provides an efficient vehicle for stable transfer of foreign genes into plant cells, a direct gene transfer method has the advantage of requiring no particular vector construction and the risk of obtaining chimaeras due to the use of multicelluar explants can be minimized. DNA-mediated transformation of protoplasts also allows the integration of a large number of foreign genes into the host genome and to a certain extent influences the number of copies integrated (Paszkowski et al.