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Biochemical Journal 299: 381-387. , Rishell, W. A. 1991. Heritable retroviral transgenes are highly expressed in chickens. P~ceedings of the National Academy of Sciences (USA). 88: 1736-1740. G. and Robertson, S. 1989. The effects of various enzyme mixtures as silage additives on feed intake and milk production of dairy cows. S. (eds) Silage for Milk Production. Occasional symposium 23, British Grassland Society, UK. pp. 187189. R. R. 1994. Effects of enzyme-inoculant systems on BIOTECHNOLOGY FOR IMPROVED RUMEN MICROBIAL ECOSYSTEM 37 preservation and nutritive value of haycrop and com silages.
However, data are now being accumulated to indicate that, at least in some cases, these difficulties may have been overestimated. There are now several systems by which new genetic material can be introduced into rumen bacteria as plasmids, and many different approaches have been' made to the transformation of rumen bacteria, and most have shown very limited success. The technique that has proven generally useful for inserting new DNA is that of electroporation using an electrical pulse to generate openings in the cell wall, through which DNA can enter (Dower et ai, 1988).
The excretion of methane from the rumen can represent a loss of up to 15% of the digestible energy depending on the type of diet. Therefore, reducing methane 34 RAVINDER NAGPAL, ANn. KUMAR PuNIA AND KISHAN SINGH production could benefit the ruminant energetically, provided the efficiency of ruminal metabolism is not compromised. Animal trials involving agents that specifically inhibit microbial enzymes associated with methane production probably provide the mosheliable data for interpretation of the effects of inhibition of methanogenesis on digestive and animal performance parameters, indicating that a reduction in methanogenesis in the rumen can be associated with improvements in feed conversion efficiency without affecting intake (Cheryl et ai, 2003).