By Rune Matthiesen, Jakob Bunkenborg (auth.), Rune Matthiesen (eds.)
Since the publishing of the 1st version, the methodologies and instrumentation fascinated about the sphere of mass spectrometry-based proteomics has more suitable significantly. absolutely revised and multiplied, Mass Spectrometry facts research in Proteomics, moment Edition offers professional chapters on particular MS-based equipment or information research innovations in proteomics. the amount covers information research issues proper for quantitative proteomics, publish translational amendment, HX-MS, glycomics, and information alternate criteria, between different themes. Written within the hugely winning Methods in Molecular Biology sequence structure, chapters comprise short introductions to their respective matters, lists of the mandatory fabrics and reagents, step by step, conveniently reproducible laboratory protocols, and tips about troubleshooting and averting identified pitfalls.
Updated and authoritative, Mass Spectrometry information research in Proteomics, moment Edition serves as an in depth advisor for all researchers trying to additional our wisdom within the box of proteomics.
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Additional info for Mass Spectrometry Data Analysis in Proteomics
Example text
ETD [11]. , anthracene) transfer an electron to multiply protonated peptides. The transferred electron induces fragmentation of the peptide backbone in a similar to that of ECD. ECD requires that the precursor sample ions are immersed in a dense population of near-thermal electrons making it a technical challenge to use ECD in ion traps because of difficulties of keeping the low mass electrons in an RF field. The advantage of ETD compared to ECD is that it can be used in combination with mass analyzers that trap ions with radio frequency (RF) electrostatic fields such as ion traps.
The following lines are the fragment ion masses, and intensity separated by the space character. 553e0 1 .. 278e-1 1 In the pkx format which is used in the VEMS program, the MS/MS spectra are separated by an empty line. The first line in an MS/MS spectrum is precursor ion mass, intensity of precursor ion, charge state, retention time, scan number, and optional extra data about the parent ion separated by the space character. The following lines are the fragment ion masses, intensity and original charge state separated by the space character.
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