By J. Warren Evans (auth.), J. Warren Evans, Alexander Hollaender, Claire M. Wilson (eds.)
J. Warren Evans division of Animal technology Texas A&M collage university Station, Texas 77843 within the close to destiny, development of family animals for the creation of meals and fiber is poised to endure a revolution via the usage of modern breakthroughs and advances in molecular genetics, embryo manipula tions, and gene move platforms. usage of those thoughts could have a large influence on animal agriculture through development of construction effi ciency through manipulation and keep an eye on of many physiological platforms. the result may be to diminish construction bills, raise nutrition construction and caliber, and reduce nutrition charges. healthiness and health of household and different animals might be greater because of new equipment of ailment analysis, vaccine creation, and affliction prevention practices. Genetic engineering additionally deals the potential of using animals for the advance of pharmaceutical items to learn society. examine development can be en hanced through manipulation of the gene pool. The goals of this convention have been to debate the present prestige of animal bioengineering and to realistically examine the capability applica tions of present and destiny genetic applied sciences for the construction of meals and fiber to satisfy the desires of our hungry international, and to supply animal sci entists who might need to make use of bioengineering in present or destiny re seek courses with present history information about options, ap plications, and methodologies.
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Additional resources for Genetic Engineering of Animals: An Agricultural Perspective
Example text
Amino acid sequencing ot native bGH as compared to rbGH revealed a similar degree of amino-acid heterogeneity at the NH2 terminus. Native bGH was reported by previous investigators (24,25) to nave a heterogenous NH2 terminal. Since rbGH possesses the same type of heterogeneity as authentic bGH (see "Results" above), a possibility exists that the information necessary for cleavage is an inherent part of the bGH molecule and not cell type-specific. Further experiments to test the observation are proceeding.
Comparison of the RSV-LTR and CMVIE Promoters in Directing Synthesis of Bovine Growth Hormone by Cultured Rodent Cells their ment. viral rials duced We were interested in comparing the RSV-LTR and the CMVIE promoters in ability to direct bGH gene expression in a similar host cell environRecombinant plasmid DNA molecules were constructed that contain promoters ligated to the coding sequence of the bGH gene (see "Mateand Methods" above). pbGH-4 and pCMVIE-bGH plasmid DNAs were introtransiently into cultured rat GH3 or mouse L cells.
Bovine GH (bGH) expression in transiently transfected mouse L cells. ) or pCMVIE-bGH ( cr--o) DNA were transfected into mouse L cells using the DEAE-Dextran-dimethyl sulfoxide shock procedure as described in "Materials and Methods" section in text. Bovine GH expression was determined as described in the legend to Fig. 8. Amino acid sequencing ot native bGH as compared to rbGH revealed a similar degree of amino-acid heterogeneity at the NH2 terminus. Native bGH was reported by previous investigators (24,25) to nave a heterogenous NH2 terminal.