Download Galectins: Methods and Protocols by Sean R. Stowell, Richard D. Cummings PDF

By Sean R. Stowell, Richard D. Cummings

Galectins: tools and Protocols is the 1st booklet exclusively devoted to methodological ways designed to check galectin functionality. The galectin family members represents the most pleiotropic households, with person participants having been implicated in a number of features of approximately each organic method defined, from RNA splicing to complicated regulatory circuits that orchestrate adaptive immunity. Given the various roles of galectins in quite a few organic platforms, learning those glycan binding proteins usually calls for the assimilation of various technical abilities to completely savour their organic functionality. Their approximately ubiquitous expression and talent to bind hugely modifiable carbohydrate ligands, as well as various different regulatory proteins, permits those glycan binding proteins (GBPs) to own the ability to manage a large choice of organic methods. person chapters are devoted to studying salient beneficial properties of galectin features. Written within the profitable Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, with no trouble reproducible protocols, and notes on troubleshooting and keeping off recognized pitfalls.

Authoritative and simply obtainable, Galectins: tools and Protocols seeks to serve either pros and beginners with an invaluable framework while studying galectin functionality for a few years to come.

Show description

Read or Download Galectins: Methods and Protocols PDF

Similar biochemistry books

Basic concepts in biochemistry: A student survival guide

This moment variation maintains to innovatively evaluate the hardest options in biochemistry for optimum comprehension in a brief time period. in contrast to traditional texts or evaluate books that pressure memorizing proof, uncomplicated options stresses the learning of primary strategies, in order that the reader actually comprehends the cloth and feels cozy utilizing it.

Biomembranes Part Q: ATP-Driven Pumps and Related Transport: Calcium, Proton, and Potassium Pumps

The delivery volumes of the Biomembranes sequence have been initiated with Volumes one hundred twenty five and 126 of tools in Enzymology. those volumes lined delivery in micro organism, Mitochondria, and Chloroplasts. Volumes 156 and 157 hide ATP-Driven Pumps and similar shipping. the subject of organic membrane delivery is a truly well timed one simply because a robust conceptual foundation for its realizing now exists

Ligand Design in Medicinal Inorganic Chemistry

Expanding the efficiency of healing compounds, whereas restricting side-effects, is a standard aim in medicinal chemistry. Ligands that successfully bind steel ions and in addition comprise particular beneficial properties to reinforce focusing on, reporting, and total efficacy are riding innovation in components of ailment analysis and treatment.

Additional resources for Galectins: Methods and Protocols

Example text

J Immunol 188(7):3127– 3137. 1103433 Cedeno-Laurent F, Barthel SR, Opperman MJ, Lee DM, Clark RA, Dimitroff CJ (2010) Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation. J Immunol 185(8):4659–4672. 1000715 Garin MI, Chu CC, Golshayan D, CernudaMorollon E, Wait R, Lechler RI (2006) Galectin-1: a key effector of regulation mediated by CD4 + CD25+ T cells. Blood 109(5): 2058–2065 Juszczynski P, Ouyang J, Monti S, Rodig SJ, Takeyama K, Abramson J, Chen W, Kutok JL, Rabinovich GA, Shipp MA (2007) The AP1dependent secretion of galectin-1 by Reed Sternberg cells fosters immune privilege in classical Hodgkin lymphoma.

Galectins, first called S-type lectins secondary to the requirement of several galectins for reduced thiols to maintain carbohydrate recognition activity, can form intramolecular and intermolecular disulfide bridges that often result in significant conformational changes that preclude carbohydrate recognition. As monomers appear to be a key intermediate in oxidative inactivation and carbohydrates can drive dimerization, ligand appears to reduce oxidative inactivation by facilitating dimer formation (Kd1 < Kd2).

1202814 73. Miko E, Meggyes M, Bogar B, Schmitz N, Barakonyi A, Varnagy A, Farkas B, Tamas P, Bodis J, Szekeres-Bartho J, Illes Z, Szereday L (2013) Involvement of galectin-9/TIM-3 pathway in the systemic inflammatory response in early-onset preeclampsia. PLoS One 8(8):e71811. 1371/journal. 0071811 74. Medzhitov R, Janeway CA Jr (1997) Innate immunity: impact on the adaptive immune response. Curr Opin Immunol 9(1):4–9 75. Karlsson A, Christenson K, Matlak M, Bjorstad A, Brown KL, Telemo E, Salomonsson E, Leffler H, Bylund J (2009) Galectin-3 functions as an opsonin and enhances the macrophage clearance of apoptotic neutrophils.

Download PDF sample

Rated 4.44 of 5 – based on 12 votes