Download Biochemistry and Molecular Biology by J. F. Theis, C. S. Newlon (auth.), Prof. Dr. Robert Brambl, PDF

By J. F. Theis, C. S. Newlon (auth.), Prof. Dr. Robert Brambl, Prof. Dr. George A. Marzluf (eds.)

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1991). In addition to the plasmid mutations described above (see ARS structure) they found mutations in five chromosomalloci called RARI-RAR5, for regulation of autonomous replication. Three have been examined in detail. RARI is an essential 19 gene whose predicted product shows no striking similarity to other proteins (Kearsey and Edwards 1987). RAR3 and RAR5 turned out to be previously isolated genes. RAR3 is the same as GALII I SPT13, and RAR5 is DST2 (Kipling et al. 1991). DST2 encodes DNA strand transfer pro tein ß, an activity presumably involved in recombination (Dykstra et al.

RAR3 is the same as GALII I SPT13, and RAR5 is DST2 (Kipling et al. 1991). DST2 encodes DNA strand transfer pro tein ß, an activity presumably involved in recombination (Dykstra et al. 1991), and the gene has also been identified as an exoribonuclease and in a screen for genes that enhance the nuclear fusion defects of karl mutants of budding yeast (reviewed by Kearsey and Kipling 1991). How RAR5 affects replication is unclear, though the plasmid stabilization phenotype results from inactivation of the gene.

Mol Cell BioI14:1796-1805 Yeast Chromosome Replieation Campbell JL, Newlon CS (1991) Chromosomal DNA replication. In Broaeh JR, Pringle JR, Jones E (eds) The moleeular and eellular biology of the yeast Saccharomyces: genome dynamies, protein synthesis, and energeties. Cold Spring Harbor Lab Press, Cold Spring Harbor, pp 41-146 Celniker SE, Sweder K, Sriene F, Bailey JE, Campbell JL (1984) Deletion mutations affeeting autonomously replieating sequenee ARS1 of Saccharomyces cerevisiae. Mol Cell Biol 4:2455-2466 Chan CSM, Tye B-K (1983) A family of Saccharomyces cerevisiae repetitive autonomously replieating sequenees that have very similar genomie environments.

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