By George Wald (auth.), Helmut Langer (eds.)
This e-book is a file of a four-day Symposium at the Biochemistry and body structure of visible Pigments, which happened instantly after the VIth overseas Congress on Photobiology, held in Bochum, Federal Republic of Germany, in August 1972. This assembly, which introduced jointly approximately 50 investigators of assorted features of the visible approach, was once dedicated to the visible cells of either vertebrates and invertebrates. while the foreign Symposium at the Biochemistry of the Retina, held at Nij megen, The Netherlands, in 1968, had targeting vertebrate photoreceptors, this Symposium handled invertebrate photoreceptors in addition, in order that employees in each one box may well develop into accustomed to contemporary development within the different quarter. The papers awarded on the Symposium have been divided into six major themes, to every of which a half-day consultation was once committed. The six components of this publication, following the intro ductory lecture, basically correspond to those classes. as well as the invited con tributions, the quantity incorporates a variety of brief communications by means of different partici pants and contributions through invited contributors, who have been not able to wait. the quantity closes, as did the Symposium, with a basic dialogue, ready and moderated by means of S. L. BONTING, within which an try used to be made to combine numerous new findings, and to reconcile convinced issues of disagreement.
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Extra resources for Biochemistry and Physiology of Visual Pigments: Symposium Held at Institut fur Tierphysiologie, Ruhr-Universitat Bochum/W. Germany, August 27–30, 1972
2. KARRER, P. , R. SCHWYZER, A. NEUWIRTH: Oxydation von 4-Methyl-o-benzochinon zu cis-cis- ~-methylmuconsaureanhydrid. Helv. Chim. 1, 12101214 (1948). 3. : Zur cis-trans-Isomerierung von Carotinoiden. Acta n, 2241-2246 (1949). 4. D. ROBESON: Crystalline neoretinene b. Science 120, 219-220 (1954). 5. : The synthesis and configuration of neo-b-vitamin A and neoretinene b. Soc. TIL 2651-2652 (1956). 6. : Cis-trans isomeric carotenoids, vitamin A, and acrylpolyenes. Springer-Verlag, Wien (1962). 7.
After these treatments, which take about 3 hours at room temperature, electronmicrographs show that the outer segments are still morphologically intact. Analysis of the resulting preparations reveals a constant composition (Table I): about 360/0 of the dry weight of the membrane lyophilisates consists of protein, 870/0 of which is accounted for by rhodopsin-protein (7); about 500/0 is due to lipids and the rest comes mainly from residual water and carbohydrates. Thus the procedure outlined above, yields, without requiring the use of any detergent, reproducible preparations of maximal rhodopsin content, the spectral characteristics of which are almost as good as those of rhodopsin, chromatographically purified in detergent solution (6).
S. FAGER: The chemistry of vertebrate and invertebrate visual photoreceptors. In: Current Topics in Bioenergetics, ed. R. SANADI. Academic Press, N. Y. (1973). 4. R. WORTHINGTON: Planar liquid-like arrangement of photopigment molecules in frog retinal receptor disk membranes. J. Mol. BioI. ~, 417-439 (1969). 5. MOODY, M. F. , J. R. PARRIS: The discrimination of polarized light by octopus: Behavioral and morphological study. Z. Vergl. Physiol. 44, 268-291 (1961). 6. : unpublished observations (1972).