By Biji T. Kurien, R. Hal Scofield
This quantity covers earlier and current western blot recommendations, equivalent to diffusion blotting, slice blotting, blotting of low and high molecular weight proteins, unmarried phone blotting and automatic blotting. Written within the hugely profitable Methods in Molecular Biology sequence structure, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and tips about troubleshooting and averting recognized pitfalls.
Thorough and state of the art, Western Blotting: equipment and Protocols will function a useful reference for these attracted to extra learn into this attention-grabbing field.
Read or Download Western Blotting: Methods and Protocols PDF
Similar biochemistry books
Basic concepts in biochemistry: A student survival guide
This moment version maintains to innovatively assessment the hardest innovations in biochemistry for optimum comprehension in a brief time period. in contrast to traditional texts or overview books that pressure memorizing proof, simple options stresses the getting to know of basic suggestions, in order that the reader actually comprehends the fabric and feels cozy utilising it.
Biomembranes Part Q: ATP-Driven Pumps and Related Transport: Calcium, Proton, and Potassium Pumps
The delivery volumes of the Biomembranes sequence have been initiated with Volumes a hundred twenty five and 126 of equipment in Enzymology. those volumes coated delivery in micro organism, Mitochondria, and Chloroplasts. Volumes 156 and 157 disguise ATP-Driven Pumps and similar shipping. the subject of organic membrane delivery is a really well timed one simply because a robust conceptual foundation for its realizing now exists
Ligand Design in Medicinal Inorganic Chemistry
Expanding the efficiency of healing compounds, whereas restricting side-effects, is a standard aim in medicinal chemistry. Ligands that successfully bind steel ions and in addition contain particular good points to reinforce concentrating on, reporting, and total efficacy are using innovation in parts of disorder prognosis and treatment.
- Unusual DNA Structures: Proceedings of the First Gulf Shores Symposium, held at Gulf Shores State Park Resort, April 6–8 1987, sponsored by the Department of Biochemistry, Schools of Medicine and Dentistry, University of Alabama at Birmingham, Birmingham,
- Raman spectroscopy in biology: principles and applications
- Biochemistry and Molecular Genetics of Cancer Metastasis: Proceedings of the Symposium on Biochemistry and Molecular Genetics of Cancer Metastasis Bethesda, Maryland — March 18–20, 1985
- Biochemistry (Fourth Edition)
Additional resources for Western Blotting: Methods and Protocols
Example text
Anal Biochem 178:255–259 12. Harlow E, Lane D (1988) Immunoblotting. In: Harlow E, Lane D (eds) Antibodies. Western Blotting: An Introduction 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. A laboratory manual. Cold Spring Harbor Laboratory, NY, p 485 Renart J, Reiser J, Stark GR (1979) Transfer of proteins from gels to diazobenzyloxymethyl paper and detection with anti-sera: a method for studying antibody specificity and antigen structure. Proc Natl Acad Sci U S A 76: 3116–3120 Elkon KB, Jankowski PW, Chu JL (1984) Blotting intact immunoglobulins and other high-molecular-weight proteins after composite agarose-polyacrylamide gel electrophoresis.
The suction power of a pump connected to a slab gel dryer system was used to drive the separated polypeptides from the gel to the nitrocellulose membrane. Both low- and high-molecular-weight proteins could be transferred using this method. 1 μm) should be used when using low-molecular-weight proteins. The gel can dry out if the procedure is carried out over 45 min and in such a scenario enough buffer should be used. In some instances low-concentration polyacrylamide gels stuck to the membrane following transfer.
Heukeshoven J, Dernick R (1995) Effective blotting of ultrathin polyacrylamide gels anchored to a solid matrix. Electrophoresis 16:748–756 44. Olsen I, Wiker HG (1998) Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support. J Immunol Methods 220:77–84 45. Chen H, Chang GD (2001) Simultaneous immunoblotting analysis with activity gel electrophoresis in a single polyacrylamide gel. Electrophoresis 22:1894–1899 46.