By Nathan P. Kaplan, Nathan P. Colowick, Donald B. McCormick, Lemuel D. Wright
The seriously acclaimed laboratory commonplace, Methods in Enzymology, is without doubt one of the such a lot hugely revered guides within the box of biochemistry. considering the fact that 1955, every one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this present day - really an important booklet for researchers in all fields of lifestyles sciences
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Additional resources for Vitamins and Coenzymes Part E
Sample text
14 B o e h r i n g e r M a n n h e i m G m b h , M a n n h e i m , G e r m a n y , B i o c h e m i c a Cat. N o . 15372 E L A B (1970). [6] AFFINITY CHROMATOGRAPHYOF NAD 43 Calculation Micromole of NAD converted per ml = micromole of NADH produced = (AE/minute) x V exdxv Assay of I m m o b i l i z e d Alcohol D e h y d r o g e n a s e The principle and reagents used for the assay of free alcohol dehydrogenase are applied to that for immobilized enzyme. 1 M HC1, 1 ml Volume is made up to 30 ml with water.
29. 30. 31. 32. 33. 34. 9 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 4 °) but are u n c o r r e c t e d for temperature. F r o m C. Bernofsky and W. J. Gallagher, Anal. Biochem. 67, 611 (1975). b Eluted as a double peak in very shallow gradients. c 3'-AMP, Y,5'-cyclic A M P , and 2',Y-cyclic A M P are not resolved from each other u n d e r the p r e s e n t conditions. d Detected by the orcinol reaction [this series, Vol. 3[12]] as previously described [C. Bernofsky, Arch. Biochem. Biophys. 166, 645 (1975)].
METHODS IN ENZYMOLOGY,VOL. 66 Copyright © 1980by Academic Press, Inc. All rights of reproduction in any form reserved. ISBN 0-12-181966-3 40 NICOTINICACID: ANALOGSAND COENZYMES [6] attached to insoluble supports. Almost no literature is yet available on the p r o c e d u r e followed conversely. In one report, 8 immobilized CoA is found to be useful for binding e n z y m e s having an affinity for CoA. Some e n z y m e s when immobilized to a solid support can be an effective affinity medium for r e c o v e r y of CoA.