By Joseph D Bronzino
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Extra resources for Tissue engineering and artificial organs
Example text
Many practitioners use the term “metabolic engineering” to refer to the directed alteration and management of cellular synthetic machinery for the purpose of producing a target molecule. The first step in any transformation is selection of an appropriate host. That is, one must decide which microorganism will express the exogenous DNA. The choice can play an important role in subsequent © 2006 by Taylor & Francis Group, LLC 1-16 Tissue Engineering and Artificial Organs steps, including the overall success of transformation, the relative ease of purifying the protein product, and even as basic a concept as whether the microorganism is even capable of producing an active form of the given protein.
4 Marker Proteins . . . . . . . . . . . . . . . . . . . . . 5 Growth of Bacterial Cultures . . . . . . . . . . . . . . 6 Regulons . . . . . . . . . . . . . . . . . . . . . . . . . 7 Engineering the System. . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Introduction Bacteria are unicellular, relatively simple, and can double in very short times.
An example of the output from a gene array experiment is best viewed in color. cgi), which is one archive for genomic and expression data. It is suggested that an example be viewed while reading this and other explanatory texts. Other databases have been developed where researchers archive their array results; other sources are provided in the reference list. Ongoing work aims to improve the “chip” technology further. For example, depending on how the DNA is processed prior to binding to the surface, different false positive and negative results can occur.