By Douglas MacN. Surgenor
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A temporary increase in bile pigment excretion 110-130 days later was noted which was interpreted as indicative of the release and subsequent catabolism of hemoglobin from red cells formed in response to anemia. These findings led them to conclude that their methods gave a re producible but clinically inapplicable means of determining red blood cell survival. Shemin and Rittenberg ( 1 9 4 6 ) employed a direct method to establish the red blood cell life span at 127 days. They incorpoated glycine labeled with Ν into the synthesis of protoprophyrin which tagged limited genera tions of red cells for the entire life span.