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Mass-spectroscopy, high-performance liquid chromatography, surface plasmon resonance [SPR]) and the chip technologies (for example, gene-arrays) with their advantage of easy multiplexing capabilities, albeit with their need for fluorescence labeling and restriction to higher molecular-weight compounds like proteins and nucleic acids thus far. In comparison with the methods just described, the cantilever technology is cheap, fast, sensitive, and applicable to a broad range of compounds. The lack of multiplexing could be overcome by the application of large cantilever arrays with >1000 cantilevers per chip.
Cantilever arrays are already employed as detectors in both static and dynamic modes (8,9). Recent articles show the potential for detection of DNA hybridization (3,12), cell capture, or toxin detection (1). Integrating cantilever arrays into microfluidic channels will significantly reduce the amount of sample required (14). Attempts have been made to get data from single-cantilever experiments for DNA (15) or antibody–antigen reactions (16) or from a two-cantilever setups using different stiffnesses for the individual cantilevers (17).
1996) Biosensor based on force microscope technology. Vacuum Sci. Technol. B 14, 789–793. 2. , and Jun, M. (2002) A novel ISFET-type biosensor based on P450 monooxygenases Biosensors Bioelectronics 17, 173–179. 3. , Baller, M. , Lang, H. , et al. (2000) Translating biomolecular recognition into nanomechanics. Science 288, 316–318. 4. , Lang, H. , et al (1997) Surface stress in the selfassembly of alkanethiols on gold Science 276, 2021–2024. 5. , Gerber, C. , Gimzewski, J. , Meyer, E. -J. (1996) Thermal analysis using a micromechanical calorimeter Appl.