By David R. Idler
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Extra info for Steroids in Nonmammalian Vertebrates
Sample text
However, since it is a tedious and time-consuming assay it is probably most useful in the initial definition 2 . Steroid Methodology 27 of the plasmatic steroid content of a species and in the validation of simpler, more practicable methods of analysis. Again the procedural steps described for the quantification of a particular steroid in h u m a n blood cannot be used for blood of another species without confirmation that the procedures are a d e q u a t e . Plasma samples containing high concentrations of lipid not re moved by solvent partition may require further purification by column or thin-layer chromatography before preparation of the radioactive derivative.
Riochem. J. 5 2 , 464. Lipsett, Μ . , ed. (1965). " G a s C h r o m a t o g r a p h y of Steroids in Biological F l u i d s . " P l e n u m , New York. Lisboa, B. P . (1969). In " M e t h o d s in E n z y m o l o g y . Steroids and T e r p e n o i d s " ( R . B. ), Vol. 15, p p . 3 - 1 5 8 . A c a d e m i c Press, N e w Y o r k . M a d e r , W. , and Buck, R. R. (1952). Anal. Chem. 2 4 , 666. , and Samuels, L. T . (1969). Endocrinology 8 5 , 402. Mayes, D . , Kern, D . C , and N u g e n t , C .
C . (1943). J. Gen. Physiol. 2 6 , 325. Z i m m e r m a n n , W . (1935). Ζ. Physiol. Chem. 2 3 3 , 2 5 1 . Chapter 3 THE FUNCTIONAL MORPHOLOGY OF S T E R O I D O G E N I C TISSUES B R I A N LOFTS a n d H O W A R D A. BERN* I. I n t r o d u c t i o n II. Testis A . Reptiles and Birds B. A m p h i b i a n s C. Fishes I I I . Ovary A . M a m m a l i a n Ovary B. Birds C . Reptiles D. Amphibians E . Fishes IV. A d r e n o c o r t i c a l (Interrenal) Tissue A. A n a t o m y a n d Histology B. Histophysiology References 37 39 42 49 57 63 65 68 73 76 83 93 94 105 117 I.