Download RNA Nanotechnology and Therapeutics: Methods and Protocols by Peixuan Guo, Farzin Haque PDF

By Peixuan Guo, Farzin Haque

This quantity includes a compilation of ideas and laboratory protocols at once with regards to RNA nano know-how and its purposes in nano biotechnology and nano medication. The chapters during this booklet conceal a variety of learn equipment that may be simply comprehended and conducted in a step by step demeanour by way of graduate scholars and postdoctoral fellows from varied medical disciplines. a few of the coated subject matters contain numerous biochemical, biophysical and bioinformatics techniques for interpreting RNA buildings and homes; the right way to study the multi-step meeting strategy of RNA nano architectures; purification of multifunctional RNA nanoparticles by means of ultracentrifugation and HPLC; real-time detection of RNA nano constructs in vivo; conjugation of imaging, focusing on and healing modules to RNA scaffold; and layout and characterization of RNA-protein nanostructures. Written within the hugely profitable Methods of Molecular Biology sequence structure, chapters comprise introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, quite simply reproducible laboratory protocols and key tips about issues troubleshooting and keeping off recognized pitfalls.

Cutting-edge and useful, RNA Nanotechnology and Therapeutics: tools and Protocols propels the sphere towards new heights via attractive researchers operating within the varied fields of actual, engineering, and lifestyles sciences.

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Additional resources for RNA Nanotechnology and Therapeutics: Methods and Protocols

Example text

The visualization is achieved with a transilluminator (λ = 254 or 300 nm). 5 Two Results Can Be Observed If there are no abortive products, the RNA can be purified directly using the RNeasy Minikit (Qiagen) according to the manufacturer’s protocol. Alternatively if there are some abortive products, the transcript needs to be purified from the PAGE. For this goal, the band corresponding to the full length sRNA has to be cut from the gel, the piece of gel is ground (crushed) and eluted overnight at 37 °C under agitation with two volumes of elution Buffer (see Note 2).

7d). The combination of these two techniques, by enabling spreading in various conditions and visualization of large area with hundreds of molecules, provides a statistics for the efficiency of the self-assembly process and on the polymorphism of the structures obtained. 7 Detection of Polymeric sRNAS in Crude Extracts The presence of RNA polymer in vivo can be investigated by Northern Blot analyses, even though it should be kept in mind that the RNA could polymerize during extraction. 1 Preparation of E.

Afonin KA, Bindewald E, Yaghoubian AJ, Voss N, Jacovetty E, Shapiro BA, Jaeger L (2010) In vitro assembly of cubic RNA-based scaffolds designed in silico. Nat Nanotechnol 5:676–682 4. Chang KY, Tinoco I Jr (1994) Characterization of a “kissing” hairpin complex derived from the human immunodeficiency virus genome. Proc Natl Acad Sci U S A 91:8705–8709 5. Chen C, Zhang C, Guo P (1999) Sequence requirement for hand-in-hand interaction in formation of RNA dimers and hexamers to gear phi29 DNA translocation motor.

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