By Sameh Magdeldin
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Extra resources for Recent Advances Proteomics Research
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We found consistent underestimation of the isotope ratio measurement when lower 2H enrichment was measured, while overestimation was observed at the higher 2H enrichment (Fig. 4). Interestingly, the error was less when doubly charged ions of the same peptide were analyzed. 5772/61776 The accuracy and precision of molar percent enrichment (MPE) determinations, calculated as the fraction of the total intensity, depends on the number of isotopomers that are used in the calculation. This is due to a lower abundance of heavy isotopomers which introduce more error in MPE calculation.
The immense information collected using these “omics” sciences helps to understand the diseases mecha‐ nisms and facilitates early diagnosis of the disease, along with implementation and evaluation of personalized therapy [72]. Utilization of genomics in particular enabled the discovery of several genetic diseases. Although multiple protein biomarkers have been identified using 37 38 Recent Advances in Proteomics Research quantitative proteomics, compared to genomics, proteomics is still lagging behind as a clinical test method.
Flooding dose or primed infusion of the stable isotope labeled amino acids are utilized to study protein turnover in human studies. Multiple studies investigated advantages and disadvantages of both methods [12, 13]. With a different degree of success, both methods enhanced our understanding of total protein dynamics in different tissues and circulation. However, both methods have been associated with several problems related to the assessment of true precursor enrichment and its impact on data interpretation; in addition, experimental design typically requires inpatient tracer administration.