By Louise von Stechow
This moment version presents updated protocols concentrating on innovations for the categorical enrichment of phosphopeptides and phosphoproteins. Phosphoproteomics tools and Protocols, moment Edition publications readers via various labeling options for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho movement cytometry; and bioinformatics suggestions for phosphoproteomics information research and integration. extra protocols pay attention to the identity of kinase-substrate relationships through either excessive- and low-throughput ways. Written within the hugely profitable Methods in Molecular Biology series structure, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, with no trouble reproducible laboratory protocols, and key tips about troubleshooting and warding off identified pitfalls.
Authoritative and practical, Phosphoproteomics tools and Protocols, moment Edition goals to make sure profitable leads to the additional research of this very important field.
Read Online or Download Phospho-Proteomics: Methods and Protocols PDF
Similar biochemistry books
Basic concepts in biochemistry: A student survival guide
This moment version keeps to innovatively evaluate the hardest innovations in biochemistry for max comprehension in a quick time period. in contrast to traditional texts or overview books that tension memorizing evidence, uncomplicated innovations stresses the getting to know of primary innovations, in order that the reader actually comprehends the fabric and feels cozy employing it.
Biomembranes Part Q: ATP-Driven Pumps and Related Transport: Calcium, Proton, and Potassium Pumps
The shipping volumes of the Biomembranes sequence have been initiated with Volumes a hundred twenty five and 126 of tools in Enzymology. those volumes coated shipping in micro organism, Mitochondria, and Chloroplasts. Volumes 156 and 157 hide ATP-Driven Pumps and comparable shipping. the subject of organic membrane shipping is a really well timed one simply because a robust conceptual foundation for its knowing now exists
Ligand Design in Medicinal Inorganic Chemistry
Expanding the efficiency of healing compounds, whereas proscribing side-effects, is a typical aim in medicinal chemistry. Ligands that successfully bind steel ions and in addition contain particular beneficial properties to augment concentrating on, reporting, and total efficacy are riding innovation in parts of ailment prognosis and treatment.
- Seed Proteins: Biochemistry, Genetics, Nutritive Value
- Neglected diseases and drug discovery
- Silicon Biomineralization: Biology — Biochemistry — Molecular Biology — Biotechnology
- Lehninger Principles of Biochemistry, Fourth Edition with CDROM
Additional info for Phospho-Proteomics: Methods and Protocols
Example text
Blocking Buffer: TBS-T, 10 % (w/v) bovine serum albumin. The solution should be freshly prepared as required. 4. 0 % (w/v) BSA. The solution should be freshly prepared as required. 5. 0 % (w/v) BSA, and 300 μg/mL human cell lysate (or 520 μg/mL bacterial cell lysate) (see Note 6). 1 μM Src kinase inhibitor I [6,7-dimethoxy-N -(4-phenoxyphenyl)quinazolin-4-amine] to the reaction buffer containing the lysate of EGF-stimulated A431 cells. All reaction buffers should be prepared freshly as required and stored on ice before use.
MALDI-TOF mass spectrometer (Voyager DE STR, Applied Biosystems, CA) or equivalent. 8. MALDI-TOF/TOF mass spectrometer (4800 Proteomics Analyzer, Applied Biosystems, CA) or equivalent. 9. 8). 6 Other Materials 1. 2 μL bed volume) for solid phase reactions. 2. 5, and 2 mL). 3. Eppendorf pipette tips. 4. 8 mL internal volume (Pierce Corp, Rockford, IL). 1 Methods In-Gel Digestion After sodium dodecylsulfate-polyacrylamide electrophoresis (SDSPAGE) and gel staining (see Note 6), carry out all manipulations in a laminar flow-vented hood or equivalent.
Equipment 2. Test peptides: DAM1 phosphopeptide SFVLNPTNIGMp SKSSQGHVTK (AnaSpec, San Jose, CA); angiotensin I peptide DRVYIHPFHL (Sigma-Aldrich, St. 2 (SigmaAldrich, St. Louis, MO). 2. Bench top centrifuge Eppendorf 5415 D or equivalent. 3. Rotary mixer. 4. Modular block heater. 5. Thermomixer. 6. Savant SpeedVac concentrator. 38 Heinz Nika et al. 7. MALDI-TOF mass spectrometer (Voyager DE STR, Applied Biosystems, CA) or equivalent. 8. MALDI-TOF/TOF mass spectrometer (4800 Proteomics Analyzer, Applied Biosystems, CA) or equivalent.