By Thomas Wartmann, Gotthard Kunze (auth.), Prof. Dr. Klaus Wolf, Prof. Dr. Karin Breunig, Prof. Dr. Gerold Barth (eds.)
Most details on yeasts derives from experiments with the traditional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the full nuclear and mitochondrial genome of which has additionally been sequenced. For all different non-conventional yeasts, investigations are in growth and the speedy improvement of molecular suggestions has allowed an perception additionally right into a number of non-conventional yeasts.
In this bench guide, over 70 functional protocols utilizing 15 diverse non-conventional yeast species and also a number of protocols of normal use are defined intimately. All of those experiments at the genetics, biochemistry and biotechnology of yeasts were contributed via well known laboratories and feature been reproduced time and again. The trustworthy protocols are hence perfect additionally for undergraduate and graduate functional courses.
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Extra info for Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology: Practical Protocols
Example text
Adeninivorans LS3, LS3 transformants, 135, 135 transformants; (2) 20 μL culture medium from A. adeninivorans LS3, LS3 transformants, 135, 135 transformants; (3) protein molecular weight standard. 5 % PAA) and transferred electrophoretically on to polyvinylidene difluoride (PVDF) membranes in a “Mini Trans-Blot Transfer Cell” (Bio-Rad, USA) filled with transfer buffer at 100 mA for 18 h. The membranes are then incubated in TBST1 at 30 °C for 60 min. GFP is then immunologically detected. After two washing steps with TBST2 and once with TBS buffer (each 25 °C, 10 min) goat anti-rabbit IgG coupled to alkaline phosphatase is used as secondary antibody at 30 °C for 60 min.
Cerevisiaederived PHO5 terminator. Insertion of these expression cassettes into the SalI–ApaI sites of the basal plasmid pAL-HPH1 results in the plasmids pAL-HPH-TEF-HSA and pAL-HPH-TEF-GFP, respectively, which are used for the transformation experiments (Fig. 1B, C). Materials Strains Media – A. adeninivorans LS3 – wild-type (Kunze and Kunze 1994) – A. adeninivorans 135 – mutant with altered dimorphism characteristics (Wartmann et al. 5 % yeast extract, 2 % glucose – YEPD-agar medium: YEPD medium with 2 % agar – One litre of YMM medium (Tanaka et al.
Zbl Mikrobiol 145:3–12 Kunze I, Kunze G (1994) Characterization of Arxula adeninivorans from different habitats. Antonie van Leeuwenhoek 65:29–34 Kunze G, Kunze I (1996) Arxula adeninivorans. In: Wolf K (ed) Nonconventional yeasts. Springer, Berlin Heidelberg New York, pp 389–409 Tanaka A, Ohnishi N, Fukui S (1967) Studies on the formation of vitamins and their function in hydrocarbon fermentation. Production of vitamins and their function in hydrocarbon medium. J Ferment Technol 45:617–623 Samsonova IA, Kunze G, Bode R, Böttcher F (1996) A set of genetic markers for the chromosomes of the imperfect yeast Arxula adeninivorans.