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70) in their sucrose feeding experiments and was demonstrated by Itoh et al. (78), who showed that plasma FFA levels begin to increase 10 hours after the last meal and rise progressively thereafter. Triglyceride levels, on the other hand, appear to be unaffected. Considerable elevation of FFA was also demonstrated by Rigas et al. (68) during starvation of obese patients, but the absence of night estimations in his work makes comment about the circadian rhythm impossible. That a rhythm is likely to be absent, however, is indicated by the lack of difference in FFA levels at 3 times of day after 28 days of starvation in the study of Marliss et al.