Download Molecular Morphology in Human Tissues: Techniques and by Gerhard W. Hacker, Raymond R. Tubbs PDF

By Gerhard W. Hacker, Raymond R. Tubbs

Molecular Morphology of Human Tissue with gentle Microscopy provides quite a few molecular morphological innovations for use in scientific diagnostics, biomedical study and biotechnology. Written through specialists within the box, the booklet comprises lately said "futuristic" methodologies, akin to the three-d demonstration of complete colour microscopic photographs acquired in general vibrant box microscopy, which doesn't rely on the necessity for expensive laser-equipped equipment (such as confocal microscopes) and for the 1st time offers 3D copy of the entire spectrum of transmitted and fluorescent mild illumination.

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Additional info for Molecular Morphology in Human Tissues: Techniques and Applications (Advances in Pathology, Microscopy, & Molecular Morphology)

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1). A triple–PAP staining can also be performed using DAB with heavy metal intensification in the first staining sequence, followed by DAB alone in the second sequence, and 4-chloro-1-naphthol in the third [12]. This double–PAP technique is only useful if the two antigens in question are localized in different cells. When the antigens are present in the same cell, significant problems can appear in evaluating the color product. Furthermore, when the DAB reaction product is strong, immunologic or catalytic interactions can occur with the second sequence antibodies or substrates [40].

Histochem. , 30, 27, 1982. 48. A. , Dual immunostaining procedure demonstrating neurotransmitter and neuropeptide codistribution in the same section, Am. J. , 175, 331, 1986. 23 1702_C001 11/17/04 10:51 AM Page 24 Molecular Morphology in Human Tissues: Techniques and Applications 49. M. , Multiple immunoenzyme staining techniques. Use of fluoresceinated, biotinylated and unlabeled monoclonal antibodies, J. Immunol. , 117, 45, 1989. 50. Gu, J. , Sequential use of the PAP and immunogold staining methods for the light microscopical double staining of tissue antigens, Regul.

Histochem. , 30, 691, 1982. 24 60. , Autoradiographic detection of [125I]-secondary antiserum: a sensitive light and electron microscopic labeling method compatible with peroxidase immunocytochemistry for dual localization of neural antigens, J. Histochem. , 34, 707, 1986. 61. -I. , Radioimmunocytochemistry — A novel immunocytochemical principle, J. Histochem. , 25, 1140, 1977. 62. , Radioimmunocytochemistry, in Immunocytochemistry. M. , Wright, Bristol, 1986, 99. 63. Grino, M. , An in situ hybridisation histochemistry technique allowing simultaneous visualization by the use of confocal microscopy of three cellular mRNA species in individual neurons, J.

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