By Giovanni Di Sabato (Eds.)
During this quantity, in vitro versions for the learn of lymphoid cellphone features and techniques for the learn of lymphoid mobile receptors are awarded. Lymphocyte in vitro transformation is mentioned within the first part that describes equipment for the in vitro stimulation of lymphocytes. a few detailed media for the learn of lymphocyte transformation also are mentioned. In vitro tools were very worthwhile for the knowledge of the differentiation of lymphoid cells. The by and large used of those tools are awarded within the moment part. The 3rd part bargains with the receptors of lymphoid cells
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Extra info for Immunochemical Techniques Part K: In Vitro Models of B and T Cell Functions and Lymphoid Cell Receptors
Sample text
Kaplan, this series, Vol. 108. 34 STIMULATION OF LYMPHOCYTES [3] cyte donors through the use of a mitogenic lectin and IL-2 may also be useful although these cells are restricted largely to the T helper cell category, grow relatively slowly, are subject to growth crises, and generally are not nearly as expandable for experimental purposes as permanent lymphoblastoid cell lines. Hybrids of human T cells with human, monkey, or murine cell lines may also be quite useful as sources of lymphocyte products although these cell lines tend to be unstable and require careful monitoring.
Cytol. 39, 89 (1974). 3 H. Lis and N. Sharon, in "The Antigens" (M. ), Vol. 4, p. 429. Academic Press, New York, 1977. METHODS IN ENZYMOLOGY, VOL. 150 Copyright © 1987 by Academic Press, Inc. All rights of reproduction in any form reserved. 18 STIMULATION OF LYMPHOCYTES [2] lymphocytes, leading to cell division, lymphokine production, and cellular cytotoxicity. 4 At physiological pH, Con A is a tetramer composed of four identical subunits and each subunit has one saccharide-binding site. Therefore Con A is a tetravalent molecule at physiological pH.
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