Download Genome Sequencing Technology and Algorithms by Sun Kim, Haixu Tang, Elaine R. Mardis PDF

By Sun Kim, Haixu Tang, Elaine R. Mardis

The 2003 final touch of the Human Genome venture used to be only one step within the evolution of DNA sequencing. Now from a "who's who" of pioneers within the box comes the newest genome sequencing and meeting advances which are redefining the sphere. This trail-blazing e-book offers researchers, unheard of entry to state of the art DNA sequencing applied sciences, new algorithmic series meeting innovations, and rising equipment for either resequencing and genome research that jointly shape the main reliable origin attainable for tackling experimental and computational demanding situations within the genome sciences this present day. together with reviews of present recommendations, this far-reaching source bargains researchers tips in attaining extra swift and actual DNA sequencing and constructing the subsequent iteration of high-throughput equipment and units.

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After digestion, the target DNA fragments are labeled and then hybridized to the RA overnight. The chip is then washed and scanned, which generates a high-definition image of the RA. At this point, the chip image is ready to be analyzed. 4 Analyzing Resequencing Array Data with ABACUS Determining the DNA sequence from a RA requires analysis of the chip image for each queried base. Initial studies using high-density oligonucleotide microarrays used for single nucleotide polymorphism (SNP) discovery and genotyping relied on manual examination and visual inspection of the chip images by the human eye [13–20].

42 Genome Sequencing Technology and Algorithms [30] Sharp, A. , “Segmental Duplications and Copy-Number Variation in the Human Genome,” Am. J. Hum. , Vol. 77, 2005, pp. 78–88. , and D. Kulp, “Model-P: A Basecalling Method for Resequencing Microarrays of Diploid Samples,” Bioinformatics, Vol. 21, Suppl. 2, 2005, pp. ii182–ii189. , Vol. 34, 2006, pp. D556–D561. 4 Polony Sequencing Jay Shendure, Gregory J. Porreca, and George M.

Morgan, and A. Patrinos, “The Human Genome Project: Lessons from Large-Scale Biology,” Science, Vol. 300, 2003, pp. 286–290. [3] Venter, J. , “The Sequence of the Human Genome,” Science, Vol. 291, 2001, pp. 1304–1351. , “Advanced Sequencing Technologies: Methods and Goals,” Nat. Rev. , Vol. 5, 2004, pp. 335–344. [5] Cutler, D. , Vol. 11, 2001, pp. 1913–1925. [6] Zwick, M. , Vol. 6, 2005, p. R10. [7] Lipshutz, R. , “High Density Synthetic Oligonucleotide Arrays,” Nat. , Vol. 21, 1999, pp. 20–24.

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