By M. A. Hayat
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Additional info for Fixation for Electron Microscopy
Sample text
It is emphasized that although the influx or outflux of a nonelectrolyte is related to its concentration at the opposite side of the membrane, the fluxes of ions are generally not such simple functions of the concentrations, but depend also on the potential difference across the membrane and on the ionic pumps in the membrane. Because of their extremely slow rate of penetration through membranes, nonelectrolytes may also act as osmotic stabilizers where the permeability properties of membranes persist, as, for example, after fixation with glutaraldehyde.
27 Osmolarity However, an agreement on the inhibitory effect of sucrose and the nature of this effect on enzyme activity is lacking. Evidence is available from both eukaryotic (Hayashi and Freiman, 1966) and prokaryotic (Burnham and Hageage, 1973) organisms that indicates that the addition of sucrose to the aldehyde fixative solutions reduces their inhibitory effect on enzyme activity. Moreover, according to Burnham and Hageage (1973), the increase in enzyme activity is possibly due to the increased osmolarity imparted to the fixative solution by the addition of sucrose.
Glycogen granules clumped. Organelles closely packed in cytoplasm. Bile canaliculi less patent. Nuclear pores not usually obvious Comparable to phosphate. Nuclear membrane less distinct with little suggestion of pores Usually easier than phosphate Uniform staining with lead but less intense than phosphate. Chromatin stains intensely with uranyl acetate Veronal acetate Noticeably more difficult than scollidine. Better with higher concentration of O s 0 4 Stains well with lead and uranyl acetate Chromatindichromate Similar to NaHCO s Inherent contrast better than with other buffers.