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Hoboken. C. T. (1996) Polymer Microscopy, Chapman & Hall, London. Scheirs, J. (2000) Compositional and Failure Analysis of Polymers: A Practical Approach, John Wiley & Sons, Ltd, Chichester. M¨uller, M. (2006) Introduction to Confocal Fluorescence Microscopy, 2nd edn, The International Society for Optical Engineering, Bellingham, WA. 7. R. N. (2002) Microscopy Techniques for Materials Science, Woodhead Publishing Ltd, Cambridge. S. and Bracegirdle, B. (1998) Introduction to Light Microscopy, BIOS Scientific Publishers, Oxford.
2 Three-Dimensional Images The technique of confocal microscopy can be considered as optical sectioning. A three-dimensional (3D) image is obtained by reconstructing a deck of plane images. 47 shows an example of how a 3D image is formed with confocal fluorescence microscopy. A biological specimen, a Spathiphyllum pollen grain, was fluorescently labeled with acridine orange. In total, 80 sections of the pollen grain were imaged for 3D reconstruction. 1 μm. 47 shows 11 of 80 plane images and the reconstructed 3D image.
The direction of rotation of the holder and the compressive force between specimen and grinding wheel can also be altered. The machines usually use running water as the cooling medium during grinding to avoid friction heat and to remove loose abrasives that are produced continuously during grinding. 2 Polishing Polishing is the last step in producing a flat, scratch-free surface. After being ground to a 600-grit finish, the specimen should be further polished to remove all visible scratches from grinding.