By Shelley D. Minteer
This quantity introduces the reader to the sphere of enzyme stabilization and the several theories of enzyme stabilization, together with using immobilization as a stabilization approach. the 1st a part of the booklet makes a speciality of protocols for enzyme stabilization in suggestions together with liposome formation, micelle advent, crosslinking, and ingredients. the second one a part of the publication discusses protocols for enzyme stabilization in the course of enzyme immobilization, together with universal strategies like sol-gel encapsulation, polymer encapsulation, and unmarried enzyme nanoparticle formation. Protocols for various enzymes are proven, however the enzymes are selected as examples to teach that those protocols can be utilized for either enzymes of organic value, in addition to enzymes of commercial value. the ultimate half information spectroscopic protocols, equipment, and assays for learning the effectiveness of the enzyme stabilization and immobilization innovations. Written within the hugely winning Methods in Molecular Biology series structure, chapters comprise introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, effectively reproducible laboratory protocols, and pointers on troubleshooting and heading off recognized pitfalls.
state-of-the-art and thorough, Enzyme Stabilization and Immobilization: equipment and Protocols, moment Edition presents molecular biologists, biochemists, and biomedical and biochemical engineers with the state of the art technical info required to successfully stabilize their enzyme of curiosity in numerous environments (i.e., harsh temperature, pH, or solvent conditions).
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Additional info for Enzyme Stabilization and Immobilization: Methods and Protocols
Example text
Vortexers or other mechanical mixers are suggested. 4. Simply pipetting the buffer into the reverse micelle solution is not sufficient for incorporation of the protein. It is important for vigorous mixing to occur. Vortexers or other mechanical mixers are suggested. 5. This solution should become clear within 1 min of gentle shaking or there is a problem with the reverse micelle preparation. References 1. Martinek K, Klyachko NL, Kabanov AV, Khmel’nitskii YL, Levashov AV (1989) Micellar enzymology: its relation to membranology.
Can J Biochem 45:839–851 6. Shome A, Roy S, Das PK (2007) Nonionic surfactants: a key to enhance the enzyme activity at cationic reverse micellar interface. Langmuir 23:4130–4136 7. Gebicka L, Jurgas-Grudzinska M (2004) Activity and stability of catalase in nonionic micellar and reverse miceller systems. Z Naturfosch 59c:887–891 8. Kitto GB, Wasserman PM, Michjeda J, Kaplan NO (1966) Multiple forms of mitochondrial malate dehydrogenases. Biochem Biophys Res Commun 22:75–81 Chapter 4 Lipase Activation and Stabilization in Room-Temperature Ionic Liquids Joel L.
Mitochondrial malate dehydrogenase can also be isolated from whole pig heart according to a method in Ref. [8]. 2. Nitrophenyloctanoate can also be synthesized as described in Ref. [6]. 3. Simply pipetting the buffer into the AOT solution will not form micelles. It is important for vigorous mixing to occur. Vortexers or other mechanical mixers are suggested. 4. Simply pipetting the buffer into the reverse micelle solution is not sufficient for incorporation of the protein. It is important for vigorous mixing to occur.