By F. Mayer (Eds.)
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A. and Homo, J. C. (1982). J. Microsc. 128, 219-237. , McDowall, A. , Schmid, E. N. and Lickfeld, K. G. (l983),J. Bacteriol. 155, 381-390. , Lepault, J. and McDowall, A. W. (1987). A. Steinbrecht and K. Zierold, eds), pp. 114-131. Springer-Verlag, Berlin. Ebersold, H. -L. and Liithy, P. (1981). Arch. Microbiol. 130. 19-23. Fernandez-Moran, H. (1960). Ann. N. Y. Acad. Sci. 85,689-698. Frederik, P. , Busing, W. M. and Hax, W. M. A. In Electron Microscopy 1984, Vol I1 (A. Csanridy, P. Rohlich and D.
Fixation For thin sectioning of bacteria, Kellenberger et al. 0. A disadvantage of this fixative is the slow penetration rate (Glauert, 1974). g. formaldehyde or acrolein). One of the most commonly used procedures is the glutaraldehyde-osmium tetroxide double fixation method. The convenience and accuracy of this procedure has made it the standard fixation method in many laboratories. Care should be taken that the fixative matches the natural environmental conditions of the microorganism in terms of pH, osmolarity and ionic state.
31 33 33 33 34 34 35 35 31 37 38 39 40 43 43 43 44 44 45 45 41 47 48 49 51 52 53 53 56 51 29 METHODS IN MICROBIOLOGY VOLUME 20 ISBN &12-521520 6 Copyright 0 1988 by Academic Press Limited All rights or reproduction in any form reserved U. B. SLEYTR el al. 30 Thin section ---- I Molecular architecture ‘Freeze-fracture I I I Protein- or glycoprotein pore protein @ Membrane protein Pepti doglycan or pseudomurein Lipoprotein @@ Periplasmic gel Lipopolysaccharide + Bilayer membrane lipids 0--9 Monolayer membrane lipids @ 0 2.