By M. A. Hayat
Simple ideas For Transmission Electron Microscopy
summary: simple concepts For Transmission Electron Microscopy
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The only portion of the aorta that receives fixative is the 2-3-cm region between ligatures A and B; the left renal artery, the two internal spermatics (testicular artery), and the lumbar vessels originating from this part of the aorta. ) 52 1. C H E M I C A L F I X A T I O N Fig. 9. Schematic drawing of lateral view of arterial supply of uterine horn with fetuses (late in pregnancy). Abdominal aorta (A), curvature of last rib (LR), tuber coxae (TC). Dotted line indicates laparotomy. The perfusion cannula is inserted in the uterine artery at 1.
For further details, see Forssmann et al. (1977). , 1981). The animal is anesthetized with pentobarbitone sodium (8 mg/kg) through a permanent cannula Fixation 51 in an ear vein; this anesthesia is maintained during the operation to a total of 1 2 14 mg/kg body weight. The animal is tied to an operating table. The dorsoventral axis is tilted to the horizontal plane by supporting the belly with wooden blocks. Laparotomy is performed on the left side under local anesthesia with lidocaine-noradrenalin along the incision line shown in Fig.
Muscle (Skeletal Muscle of Rat Hind Perfusates A. Locke's solution containing 0 . 24 Limb) 46 1. 0 g NaN02 1000 ml Distilled water to make The final solution contains 1 mM CaCl 2. B. 2) to make 500 ml The final solution contains 2 mM CaCl 2 and has an osmolality of 474 mosmols. Procedure. The animal is anesthetized intraperitoneally with 40 mg of Nembutal/kg of body weight. The abdominal aorta is cannulated just above the bifurcation to the hind limbs, and the vena cava is cut to allow free flow of solutions.